Hive Novel Discourse

	Main Index Search Register Login Who's Online FAQ Links
	Main Index Search Register Login Who's Online FAQ Links

	1 Online, 0 Active		You are not logged in

This is a historical archiveThe forum is read-only. Private information has been removed. It is not possible to login.

	Novel Discourse

All 9 posts Subject: Biosynthesis of Cocaine Please login to post Down

Lem2
(Hive Bee)
04-10-02 17:20
No 295048
      Biosynthesis of Cocaine

Does anyone out there by any chance have any data on the biosynthetic pathway(s) to cocaine? I have been looking around but have found nothing as of yet.

I have been doing some reasearch recently into the incorporation of naturally occuring enzymes into organic synthesis, and SWIM is very curious about doing an extraction of the enzymes of some kind of coca plant, and seeing if SWIM can get them to produce cocaine at SWIM's leisure.

hehe, wouldn't it be fun if we could add a dash of ATP, and a splash of some amino acids, and get a little bit of cocaine! And who knows, if they can be made to produce at significant velocity, perhaps it would be a viable route to mass production! cool
He who makes a beast of himself gets rid of the pain of being a man.

Rhodium
(Chief Bee)
04-10-02 18:55
No 295082
User Picture       Cocaine biosynthetic pathway
(Rated as: excellent)

Check out the article below for the biosynthetic pathway of cocaine as it was thought to be 10 years ago - I dunno if they have refined the theory since then.

Late intermediates in the biosynthesis of cocaine: 4-(1-methyl-2-pyrrolidinyl)-3-oxobutanoate and methyl ecgonine
Edward Leete, Jeffrey A. Bjorklund, Maria M. Couladis, Sung Hoon Kim
J. Am. Chem. Soc. 113(24), 9286-9292 (1991) (https://www.rhodium.ws/pdf/cocaine.biosynthetic.pathway.pdf)

Organikum
(Hive Bee)
04-10-02 20:52
No 295135
      chinese

I saw the abstract of an chinese patent on making ephedrine by cultivation of ephedra cells in huge tubs.
It was at espacenet, sorry can´t find it now.
Big problem may be to get hold of a coca plant, if someone has an idea on this please PM or mail me. (one from JAVA would be prefered... smile ).
Why should this not work for coca, as the metabolism is fairly well known and documented. (please don´t nail me on this, it´s my estimation only).

Anyone able and willing to translate the chinese patent?
I shure would dig for it
ORGY
"I hope I'm becoming more eccentric. More room, you know.
More room in the brain."

Lem2
(Hive Bee)
04-12-02 05:03
No 295862
      hehe

hehe, time for a trip to the library then. The amount of info I have been able to find on the internet is very little.

The general usage of enzymes in the lab seems to be quite straight forward, just keep the PH at the right levels, and the reactants at the right levels, and things just happen. So unless the enzymes in the pathway throw me a curve ball, and if SWIM can get seeds/leaves of a coca plant, or hell, maybe SWIM will be able to custom order the enzymes from some biochem place(prolly not, hella expensive) SWIM just might be able to get together a lean mean, coke making machine!

If you can get the levels of reactants right, one just might be able to turn a few grams of leaves into several kg's of pure coke. you will just have to wait a week or two, depending, of course, on the rate of reaction of the enzymes.

oh, and as an aside, I always though that it would be fun to try to make a krebs cycle in a bucket or something, perhaps complete w/ mitchondria, that could turn sugar directly into an electrical charge. hehe I can just see it now, "Hey, you need to put another pound of sugar in your gastank!" tongue
He who makes a beast of himself gets rid of the pain of being a man.

Organikum
(Hive Bee)
04-12-02 05:08
No 295864
      Biosomething

I found it:

Epherdrine producing method by using large-scale ephedra cell culture as material

Patent Number: CN1256316
Publication date: 2000-06-14
Inventor(s): CHA LIHANG (CN); JIAO YUXIA (CN); LIU DALU (CN)
Applicant(s):: INST OF CHEMICAL METALLURG CHI (CN)
Requested Patent: CN1256316
Application Number: CN19980125186 19981204
Priority Number(s): CN19980125186 19981204
IPC Classification: C12P13/00
EC Classification:
EC Classification:
Equivalents: CN1077600B
Abstract
The present invention relates to the method of producing ephedrine by using large-scale ephedra cell ZHJ-25 CGMCC No.0359 culture as raw material. Ephedra cell ZHJ-25 CGMCC No.0359 is inoculated intosugar containing culture medium through three ways, and cultured in 25 +/- 2 deg.C for 20-50 days before the culture is dried at 60 deg.C to obtain ephedrine-containing raw material. The method is simple, may be used in continuous or semi-continuous industrial production of ephedrine in high yield and the product has stable ephedrine content.

Not so easy to get hold of as they also invented the spelling of ephedrine new.

All in all this might be a considerable pathway to many beloved compounds.
Not for those who are always in a hurry.

If anybody has seen something similar as patent - not written in chinese/japanese, I would be very appreciated to get a hint.

thanks
ORGY
"I hope I'm becoming more eccentric. More room, you know.
More room in the brain."

Organikum
(Hive Bee)
04-12-02 05:19
No 295871
User Picture       bioelectricity

To get bioelectricity you just have to put a dynamo on your hamsters runwheel.

hehe, time for a trip to the library then. The amount of info I have been able to find on the internet is very little.

always a good idea. Have a look for enzymes vs. hormones.
And on Coca-seeds too.

Have a nice week
ORGY
"I hope I'm becoming more eccentric. More room, you know.
More room in the brain."

Lem2
(Hive Bee)
04-12-02 07:03
No 295925
User Picture       asdf

SWEET FIND! I will definatly look into THAT patent!
He who makes a beast of himself gets rid of the pain of being a man.

Rhodium
(Chief Bee)
07-18-03 00:54
No 448138
      Cocaine biosynthetic pathway - Update

Check out Post 295082 (Rhodium: "Cocaine biosynthetic pathway", Novel Discourse) in this thread, I have updated it with a picture of the biosynthetic pathway, as well as a link to the article in question.

Rhodium
(Chief Bee)
07-27-03 22:05
No 450245
User Picture       N-Methylputrescine Oxidation in Cocaine Biosynth

N-Methylputrescine Oxidation during Cocaine Biosynthesis
Thomas R. Hoye, Jeffrey A. Bjorklund, Dmitry O. Koltun, and Matthew K. Renner
Org. Lett., 2(1), 3-5 (2000) (https://www.rhodium.ws/pdf/cocaine.biosynth.n-methylputrescine.pdf)
DOI:10.1021/ol990940s

	https://the-hive.archive.erowid.org	the-hive@erowid.org



Powdered by Human Biocomputer V. 6.15.0, (c) 2014-2021, Paranoia Group
Links Erowid Rhodium

	All 9 posts		Subject: Biosynthesis of Cocaine		Please login to post		Down


		Lem2 (Hive Bee) 04-10-02 17:20 No 295048				Biosynthesis of Cocaine
						Does anyone out there by any chance have any data on the biosynthetic pathway(s) to cocaine? I have been looking around but have found nothing as of yet. I have been doing some reasearch recently into the incorporation of naturally occuring enzymes into organic synthesis, and SWIM is very curious about doing an extraction of the enzymes of some kind of coca plant, and seeing if SWIM can get them to produce cocaine at SWIM's leisure. hehe, wouldn't it be fun if we could add a dash of ATP, and a splash of some amino acids, and get a little bit of cocaine! And who knows, if they can be made to produce at significant velocity, perhaps it would be a viable route to mass production! He who makes a beast of himself gets rid of the pain of being a man.



		Rhodium (Chief Bee) 04-10-02 18:55 No 295082				Cocaine biosynthetic pathway (Rated as: excellent)
						Check out the article below for the biosynthetic pathway of cocaine as it was thought to be 10 years ago - I dunno if they have refined the theory since then. Late intermediates in the biosynthesis of cocaine: 4-(1-methyl-2-pyrrolidinyl)-3-oxobutanoate and methyl ecgonine Edward Leete, Jeffrey A. Bjorklund, Maria M. Couladis, Sung Hoon Kim J. Am. Chem. Soc. 113(24), 9286-9292 (1991) (https://www.rhodium.ws/pdf/cocaine.biosynthetic.pathway.pdf)



		Organikum (Hive Bee) 04-10-02 20:52 No 295135				chinese
						I saw the abstract of an chinese patent on making ephedrine by cultivation of ephedra cells in huge tubs. It was at espacenet, sorry can´t find it now. Big problem may be to get hold of a coca plant, if someone has an idea on this please PM or mail me. (one from JAVA would be prefered...). Why should this not work for coca, as the metabolism is fairly well known and documented. (please don´t nail me on this, it´s my estimation only). Anyone able and willing to translate the chinese patent? I shure would dig for it ORGY "I hope I'm becoming more eccentric. More room, you know. More room in the brain."



		Lem2 (Hive Bee) 04-12-02 05:03 No 295862				hehe
						hehe, time for a trip to the library then. The amount of info I have been able to find on the internet is very little. The general usage of enzymes in the lab seems to be quite straight forward, just keep the PH at the right levels, and the reactants at the right levels, and things just happen. So unless the enzymes in the pathway throw me a curve ball, and if SWIM can get seeds/leaves of a coca plant, or hell, maybe SWIM will be able to custom order the enzymes from some biochem place(prolly not, hella expensive) SWIM just might be able to get together a lean mean, coke making machine! If you can get the levels of reactants right, one just might be able to turn a few grams of leaves into several kg's of pure coke. you will just have to wait a week or two, depending, of course, on the rate of reaction of the enzymes. oh, and as an aside, I always though that it would be fun to try to make a krebs cycle in a bucket or something, perhaps complete w/ mitchondria, that could turn sugar directly into an electrical charge. hehe I can just see it now, "Hey, you need to put another pound of sugar in your gastank!" He who makes a beast of himself gets rid of the pain of being a man.



		Organikum (Hive Bee) 04-12-02 05:08 No 295864				Biosomething
						I found it: Epherdrine producing method by using large-scale ephedra cell culture as material Patent Number: CN1256316 Publication date: 2000-06-14 Inventor(s): CHA LIHANG (CN); JIAO YUXIA (CN); LIU DALU (CN) Applicant(s):: INST OF CHEMICAL METALLURG CHI (CN) Requested Patent: CN1256316 Application Number: CN19980125186 19981204 Priority Number(s): CN19980125186 19981204 IPC Classification: C12P13/00 EC Classification: EC Classification: Equivalents: CN1077600B Abstract The present invention relates to the method of producing ephedrine by using large-scale ephedra cell ZHJ-25 CGMCC No.0359 culture as raw material. Ephedra cell ZHJ-25 CGMCC No.0359 is inoculated intosugar containing culture medium through three ways, and cultured in 25 +/- 2 deg.C for 20-50 days before the culture is dried at 60 deg.C to obtain ephedrine-containing raw material. The method is simple, may be used in continuous or semi-continuous industrial production of ephedrine in high yield and the product has stable ephedrine content. Not so easy to get hold of as they also invented the spelling of ephedrine new. All in all this might be a considerable pathway to many beloved compounds. Not for those who are always in a hurry. If anybody has seen something similar as patent - not written in chinese/japanese, I would be very appreciated to get a hint. thanks ORGY "I hope I'm becoming more eccentric. More room, you know. More room in the brain."



		Organikum (Hive Bee) 04-12-02 05:19 No 295871				bioelectricity
						To get bioelectricity you just have to put a dynamo on your hamsters runwheel. hehe, time for a trip to the library then. The amount of info I have been able to find on the internet is very little. always a good idea. Have a look for enzymes vs. hormones. And on Coca-seeds too. Have a nice week ORGY "I hope I'm becoming more eccentric. More room, you know. More room in the brain."



		Lem2 (Hive Bee) 04-12-02 07:03 No 295925				asdf
						SWEET FIND! I will definatly look into THAT patent! He who makes a beast of himself gets rid of the pain of being a man.



		Rhodium (Chief Bee) 07-18-03 00:54 No 448138				Cocaine biosynthetic pathway - Update
						Check out Post 295082 (Rhodium: "Cocaine biosynthetic pathway", Novel Discourse) in this thread, I have updated it with a picture of the biosynthetic pathway, as well as a link to the article in question.



		Rhodium (Chief Bee) 07-27-03 22:05 No 450245				N-Methylputrescine Oxidation in Cocaine Biosynth
						N-Methylputrescine Oxidation during Cocaine Biosynthesis Thomas R. Hoye, Jeffrey A. Bjorklund, Dmitry O. Koltun, and Matthew K. Renner Org. Lett., 2(1), 3-5 (2000) (https://www.rhodium.ws/pdf/cocaine.biosynth.n-methylputrescine.pdf) DOI:10.1021/ol990940s

All 9 posts	End of thread	Top