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All 3 posts Subject: Artificial cocaine biosynthesis Please login to post Down

pericles
(Stranger)
07-06-03 21:46
No 444992
      Artificial cocaine biosynthesis

The thread containing the post Post 295864 (Organikum: "Biosomething", Novel Discourse) talks about possibily adapting this method for producing ephedra to produce cocaine. Has anyone made any progress researching this option?

I'm afraid I don't know too much about microbiology (but can certainly learn), but I will likely soon have access to a quantity of erythroxylum coca plants, and would be happy to give culturing cocaine in a tub a shot if I had some pointers on what the procedure would look like.

So, questions:
* Is everyone agreed that this is at least a reasonable possibility, biologically speaking?
* Would it be completely impossible to do without industrial facilities?
* Has anyone found a translation of the patent discussed Post 295864 (Organikum: "Biosomething", Novel Discourse) here?
* Is there a particular area of biology that would focus on this topic?

I'm certainly not opposed to putting a fair bit of effort into this, as long as there's a reasonable chance of success.

pericles
(Stranger)
07-11-03 00:42
No 446290
      ephedra cell ZHJ-25 CGMCC No.0359

The patent in Organikum's post refers to "ephedra cell ZHJ-25 CGMCC No.0359". Here's the patent on it:

Title: Ephedra cell ZHJ-25 CGMCC No.0359 and its induction method
Application Number: 98125187 Application Date: 1998.12.04
Publication Number: 1256309 Publication Date: 2000.06.14
Approval Pub. Date: Granted Pub. Date:
International Classification: C12N 5/04
Applicant(s) Name: Inst. of Chemical Metallurgy, Chinese Academy of Sciences
Address: (100080)
Inventor(s) Name: Cha Lihang
Attorney & Agent: gao cunxiu
Abstract
The present invention relates to the utilization of biological technology. The induced Ephedra cell ZHJ-25 is preserved in Chinese Microbe Preservation Center in the code of CGMCC No.0359. The induction method of the present invention is t hat after cleaning and disinfection, Ephedra plant is inoculated into culture medium with 1.0-10% wt./vol.sugar culture medium for culture at 23-26 deg.c for 5-30 days to obtain Ephedra callus, which is then transferred to culture medium fo r subculture. The Ephedra cell of the present invention has a proliferation of 3-5 times and a ephedrine content of 0.1-20% dry weight.

urushibara
(Hive Bee)
07-24-03 11:56
No 449500
User Picture       omg the possibilities

perhaps this could also be adapted to other plants too - for example, peyote, mimosa hostilis, iboga... hmmm perhaps that acacia rididula or berklandii (sp?)... certainly the possibility of vat culturing fungi has been well researched and usable now.

dammit now I am really going to have to learn tissue culturing and progress from there...

we can't stop here, this is bat country

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	All 3 posts		Subject: Artificial cocaine biosynthesis		Please login to post		Down


		pericles (Stranger) 07-06-03 21:46 No 444992				Artificial cocaine biosynthesis
						The thread containing the post Post 295864 (Organikum: "Biosomething", Novel Discourse) talks about possibily adapting this method for producing ephedra to produce cocaine. Has anyone made any progress researching this option? I'm afraid I don't know too much about microbiology (but can certainly learn), but I will likely soon have access to a quantity of erythroxylum coca plants, and would be happy to give culturing cocaine in a tub a shot if I had some pointers on what the procedure would look like. So, questions: * Is everyone agreed that this is at least a reasonable possibility, biologically speaking? * Would it be completely impossible to do without industrial facilities? * Has anyone found a translation of the patent discussed Post 295864 (Organikum: "Biosomething", Novel Discourse) here? * Is there a particular area of biology that would focus on this topic? I'm certainly not opposed to putting a fair bit of effort into this, as long as there's a reasonable chance of success.



		pericles (Stranger) 07-11-03 00:42 No 446290				ephedra cell ZHJ-25 CGMCC No.0359
						The patent in Organikum's post refers to "ephedra cell ZHJ-25 CGMCC No.0359". Here's the patent on it: Title: Ephedra cell ZHJ-25 CGMCC No.0359 and its induction method Application Number: 98125187 Application Date: 1998.12.04 Publication Number: 1256309 Publication Date: 2000.06.14 Approval Pub. Date: Granted Pub. Date: International Classification: C12N 5/04 Applicant(s) Name: Inst. of Chemical Metallurgy, Chinese Academy of Sciences Address: (100080) Inventor(s) Name: Cha Lihang Attorney & Agent: gao cunxiu Abstract The present invention relates to the utilization of biological technology. The induced Ephedra cell ZHJ-25 is preserved in Chinese Microbe Preservation Center in the code of CGMCC No.0359. The induction method of the present invention is t hat after cleaning and disinfection, Ephedra plant is inoculated into culture medium with 1.0-10% wt./vol.sugar culture medium for culture at 23-26 deg.c for 5-30 days to obtain Ephedra callus, which is then transferred to culture medium fo r subculture. The Ephedra cell of the present invention has a proliferation of 3-5 times and a ephedrine content of 0.1-20% dry weight.



		urushibara (Hive Bee) 07-24-03 11:56 No 449500				omg the possibilities
						perhaps this could also be adapted to other plants too - for example, peyote, mimosa hostilis, iboga... hmmm perhaps that acacia rididula or berklandii (sp?)... certainly the possibility of vat culturing fungi has been well researched and usable now. dammit now I am really going to have to learn tissue culturing and progress from there... we can't stop here, this is bat country

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