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All 6 posts Subject: 5 Color Test Reagents for Drug Identifiation Please login to post Down

embezzler
(Hive Addict)
04-13-04 20:01
No 500634
User Picture       5 Color Test Reagents for Drug Identifiation

The following is taken from Saffersteins "criminalistics" an introduction to forensic science, ISBN 0-13-013827-4   , pages 249-50.

The five primary colour test reagents are:

1. marquis (2 per cent formaldehyde in h2so4). the reagent turns purple in the presence of heroin and morphine and most opium derivatives.
Marquis will also turn brown when mixed with amphetamines and methamphetamines.

2. Dillie-koppanyi (1 % cobalt acetate in MEOH is first added to the material, followed by a 5% isopropylamine in methanol). this is a valuable test for barbituates, in whose presence the reagent turns voilet-blue in colour.

3. Duquenois-Levine (sol. A is a mixture of 2% vanillin and 1% acetaldehyde in ethyl alcohol solution; solution B is conc. hcl; solution C is chloroform). this is a valuable colour test for marijuana performed by adding solutions a,b and c respectively, to the suspect vegetation.A posative result is shown by a purple colour in the chloroform layer.

4. Van urk (1% solution of p-dimethylaminobenzaldehyde in 10% conc hcl and ethyl alcohol). Reagent turns blue-purple in the presence of lsd.however due to the small quantaties of lsd in illicit preperationsthis test is difficult to carry out under field conditions.

5. Scott test (solution A is a 2% cobalt thiocyanate dissolved in water and glycerine[1:1] solution;solution b is conc. hcl; solution c is chloroform). this is a colour test for cocaine. A powder containing cocaine will turn sol. a blue.Upon addition of B, the blue colour is transformed to a clear pink colour.upon addition of c, if cocaine is present, the blue colour reappears in the chloroform layer.

swim wonders if the Marquis could be a simple ephedrine test,seeing as how the reagent isnt too difficult to make??
the test reagents that are easier to make may help bees who come across street drugs regularly.

chemically enhanced.

gsus
(Hive Bee)
04-14-04 01:44
No 500711
      not according to the feds

Color Test Reagents/Kits for Preliminary Identification of Drugs of Abuse
U.S. Department of Justice
NIJ Standard–0604.01 (https://www.rhodium.ws/pdf/colortestreference.pdf)

http://www.druglibrary.org/toc.htm

Rhodium
(Chief Bee)
07-29-04 21:03
No 522536
User Picture       12 Chemical spot tests for the detection of drugs
(Rated as: excellent)

Validation of twelve chemical spot tests for the detection of drugs of abuse
Carol L. O’Neal, Dennis J. Crouch, and Alim A. Fatah
Forensic Science International 109(3), 189-201 (2000) (https://www.rhodium.ws/pdf/forensic/drug.spot.test.validation.pdf)

Abstract
Validation procedures are described for 12 chemical spot tests including cobalt thiocyanate, Dille–Koppanyi, Duquenois–Levine, Mandelin, Marquis, nitric acid, para-dimethylaminobenzaldehyde, ferric chloride, Froehde, Mecke, Zwikker and Simon’s (nitroprusside). The validation procedures include specificity and limit of detection. Depending on the specificity of each color test, between 28 to 45 drugs or chemicals were tested in triplicate with each of the 12 chemical spot tests. For each chemical test, the final colors resulting from positive reactions with known amounts of analytes were compared to two reference color charts. For the identification of unknown drugs, reference colors from the Inter-Society Color Council and the National Bureau of Standards (ISCC-NBS) and Munsell charts are included along with a description of each final color. These chemical spot tests were found to be very sensitive with limits of detection typically 1 to 50 µg depending on the test and the analyte.

^{The Hive - Clandestine Chemists Without Borders}

demorol
(Hive Bee)
07-30-04 15:28
No 522717
      Re: 4. Van urk (1% solution of ...

4. Van urk (1% solution of p-dimethylbenzaldehyde in 10% conc hcl and ethyl alcohol).

I think p-dimethylaminobenzaldehyde is used in this reaction rather than p-dimethylbenzaldehyde.

methyl_ethyl
(Guardian)
07-30-04 17:32
No 522735
User Picture       Correct

I think p-dimethylaminobenzaldehyde is used in this reaction rather than p-dimethylbenzaldehyde.

Correct

There are quite a few variations of the Van Urk's reagent, I have always referred to it as Ehrlich's reagent. Rhodi's post describes a 2% p-dmab, 50/50 ethanolic/conc.hydrochloric solution, I have also heard of using sulfuric acid, and using p-dmab in concentrations up to 10%, along with the omission of the alcohol.

There is something addicting about the smell of p-dmab. It is very alluring, an amine and an aldehyde together as one smile

Of course I would not suggest sniffing this stuff as it is quite irritating.

   Unipolar Mania, It's good for life... laugh

Rhodium
(Chief Bee)
09-02-04 08:07
No 529077
User Picture       ID of Codeine & Antidepressants using TLC
(Rated as: good read)

Idendification of the drugs in mixtures using thin-layer chromatography in sudden poisoning cases
Kazlauskiene D, Vainauskas P, Rakauskaite D; Medicina (Kaunas), 39 [Suppl 2] 132-6 (2003)
Medline (PMID=14617874)

Abstract
The problem of acute intoxication has become very urgent now due to a great number of various chemical preparations accumulated during the last decades in the environment. Intoxications with psychotropic drugs and their mixtures form the significant part of the intoxications; there is an increasing tendency of intoxication with several preparations at a time. Amitriptyline and codeine are the preparations, which more frequently can cause intoxication. Fluoxetine is one of the newest and often used antidepressants. Under certain circumstances, like overdose, using all preparations together, long term using or using for suicide, these preparations can be even a cause of death. In such cases amitriptyline, fluoxetine and codeine become the objects of chemical-toxicological analysis. The possibility of the separation and identification of amitriptyline, fluoxetine and codeine in the mixture using thin-layer chromatography was established.

Dragendorf reagent, modified by Munje, is most suitable for the spray-distinct of the chromatographic plates for all three substances. Amitriptyline research limit, using this developer, is 0.4 micro g, fluoxetine - 1.6 micro g, codeine - 0.8 micro g. Most acceptable for separation the components of the mixture are 5 mobile phases:

1. Diethyl acetate-methanol-ammonium hydroxide (concentrated solution) (85:10:5). Amitriptyline, fluoxetine and codeine R(f) medium values respectively are 0.94; 0.63; 0.51.
2. Buthylacetate-methanol-ammonium hydroxide (concentrated solution) (85:10:5). Amitriptyline, fluoxetine and codeine R(f) medium values respectively are 0.65; 0.24; 0.15.
3. Cyclohexane-diethyl acetate-diethyl amine (70:15:15). Amitriptyline, fluoxetine and codeine R(f) medium values respectively are 0.93; 0.75; 0.37.
4. Cyclohexane-buthylacetate-diethyl amine (70:15:15). Amitriptyline, fluoxetine and codeine R(f) medium values respectively are 0.92; 0.51; 0.25.
5. Acetone-1,4-dioxane-ammonium hydroxide (concentrated solution) (30:68:2). Amitriptyline, fluoxetine and codeine R(f) medium values respectively are 0.82; 0.62; 0.42.

Recommended methodology for the separation and identification of amitriptyline, fluoxetine and codeine in the mixture using thin-layer chromatography is statistically reliable: when confidence level is 0.95, relative error is less than 0.05; standard deviation is from 0.007 to 0.03. Recommended methodology suits for mixture, extracted from biological liquids, components separation and identification.

^{The Hive - Clandestine Chemists Without Borders}

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	All 6 posts		Subject: 5 Color Test Reagents for Drug Identifiation		Please login to post		Down


		embezzler (Hive Addict) 04-13-04 20:01 No 500634				5 Color Test Reagents for Drug Identifiation
						The following is taken from Saffersteins "criminalistics" an introduction to forensic science, ISBN 0-13-013827-4 , pages 249-50. The five primary colour test reagents are: 1. marquis (2 per cent formaldehyde in h2so4). the reagent turns purple in the presence of heroin and morphine and most opium derivatives. Marquis will also turn brown when mixed with amphetamines and methamphetamines. 2. Dillie-koppanyi (1 % cobalt acetate in MEOH is first added to the material, followed by a 5% isopropylamine in methanol). this is a valuable test for barbituates, in whose presence the reagent turns voilet-blue in colour. 3. Duquenois-Levine (sol. A is a mixture of 2% vanillin and 1% acetaldehyde in ethyl alcohol solution; solution B is conc. hcl; solution C is chloroform). this is a valuable colour test for marijuana performed by adding solutions a,b and c respectively, to the suspect vegetation.A posative result is shown by a purple colour in the chloroform layer. 4. Van urk (1% solution of p-dimethylaminobenzaldehyde in 10% conc hcl and ethyl alcohol). Reagent turns blue-purple in the presence of lsd.however due to the small quantaties of lsd in illicit preperationsthis test is difficult to carry out under field conditions. 5. Scott test (solution A is a 2% cobalt thiocyanate dissolved in water and glycerine[1:1] solution;solution b is conc. hcl; solution c is chloroform). this is a colour test for cocaine. A powder containing cocaine will turn sol. a blue.Upon addition of B, the blue colour is transformed to a clear pink colour.upon addition of c, if cocaine is present, the blue colour reappears in the chloroform layer. swim wonders if the Marquis could be a simple ephedrine test,seeing as how the reagent isnt too difficult to make?? the test reagents that are easier to make may help bees who come across street drugs regularly. chemically enhanced.



		gsus (Hive Bee) 04-14-04 01:44 No 500711				not according to the feds
						Color Test Reagents/Kits for Preliminary Identification of Drugs of Abuse U.S. Department of Justice NIJ Standard–0604.01 (https://www.rhodium.ws/pdf/colortestreference.pdf) http://www.druglibrary.org/toc.htm



		Rhodium (Chief Bee) 07-29-04 21:03 No 522536				12 Chemical spot tests for the detection of drugs (Rated as: excellent)
						Validation of twelve chemical spot tests for the detection of drugs of abuse Carol L. O’Neal, Dennis J. Crouch, and Alim A. Fatah Forensic Science International 109(3), 189-201 (2000) (https://www.rhodium.ws/pdf/forensic/drug.spot.test.validation.pdf) Abstract Validation procedures are described for 12 chemical spot tests including cobalt thiocyanate, Dille–Koppanyi, Duquenois–Levine, Mandelin, Marquis, nitric acid, para-dimethylaminobenzaldehyde, ferric chloride, Froehde, Mecke, Zwikker and Simon’s (nitroprusside). The validation procedures include specificity and limit of detection. Depending on the specificity of each color test, between 28 to 45 drugs or chemicals were tested in triplicate with each of the 12 chemical spot tests. For each chemical test, the final colors resulting from positive reactions with known amounts of analytes were compared to two reference color charts. For the identification of unknown drugs, reference colors from the Inter-Society Color Council and the National Bureau of Standards (ISCC-NBS) and Munsell charts are included along with a description of each final color. These chemical spot tests were found to be very sensitive with limits of detection typically 1 to 50 µg depending on the test and the analyte. ^{The Hive - Clandestine Chemists Without Borders}



		demorol (Hive Bee) 07-30-04 15:28 No 522717				Re: 4. Van urk (1% solution of ...
						4. Van urk (1% solution of p-dimethylbenzaldehyde in 10% conc hcl and ethyl alcohol). I think p-dimethylaminobenzaldehyde is used in this reaction rather than p-dimethylbenzaldehyde.



		methyl_ethyl (Guardian) 07-30-04 17:32 No 522735				Correct
						I think p-dimethylaminobenzaldehyde is used in this reaction rather than p-dimethylbenzaldehyde. Correct There are quite a few variations of the Van Urk's reagent, I have always referred to it as Ehrlich's reagent. Rhodi's post describes a 2% p-dmab, 50/50 ethanolic/conc.hydrochloric solution, I have also heard of using sulfuric acid, and using p-dmab in concentrations up to 10%, along with the omission of the alcohol. There is something addicting about the smell of p-dmab. It is very alluring, an amine and an aldehyde together as one Of course I would not suggest sniffing this stuff as it is quite irritating. Unipolar Mania, It's good for life...



		Rhodium (Chief Bee) 09-02-04 08:07 No 529077				ID of Codeine & Antidepressants using TLC (Rated as: good read)
						Idendification of the drugs in mixtures using thin-layer chromatography in sudden poisoning cases Kazlauskiene D, Vainauskas P, Rakauskaite D; Medicina (Kaunas), 39 [Suppl 2] 132-6 (2003) Medline (PMID=14617874) Abstract The problem of acute intoxication has become very urgent now due to a great number of various chemical preparations accumulated during the last decades in the environment. Intoxications with psychotropic drugs and their mixtures form the significant part of the intoxications; there is an increasing tendency of intoxication with several preparations at a time. Amitriptyline and codeine are the preparations, which more frequently can cause intoxication. Fluoxetine is one of the newest and often used antidepressants. Under certain circumstances, like overdose, using all preparations together, long term using or using for suicide, these preparations can be even a cause of death. In such cases amitriptyline, fluoxetine and codeine become the objects of chemical-toxicological analysis. The possibility of the separation and identification of amitriptyline, fluoxetine and codeine in the mixture using thin-layer chromatography was established. Dragendorf reagent, modified by Munje, is most suitable for the spray-distinct of the chromatographic plates for all three substances. Amitriptyline research limit, using this developer, is 0.4 micro g, fluoxetine - 1.6 micro g, codeine - 0.8 micro g. Most acceptable for separation the components of the mixture are 5 mobile phases: 1. Diethyl acetate-methanol-ammonium hydroxide (concentrated solution) (85:10:5). Amitriptyline, fluoxetine and codeine R(f) medium values respectively are 0.94; 0.63; 0.51. 2. Buthylacetate-methanol-ammonium hydroxide (concentrated solution) (85:10:5). Amitriptyline, fluoxetine and codeine R(f) medium values respectively are 0.65; 0.24; 0.15. 3. Cyclohexane-diethyl acetate-diethyl amine (70:15:15). Amitriptyline, fluoxetine and codeine R(f) medium values respectively are 0.93; 0.75; 0.37. 4. Cyclohexane-buthylacetate-diethyl amine (70:15:15). Amitriptyline, fluoxetine and codeine R(f) medium values respectively are 0.92; 0.51; 0.25. 5. Acetone-1,4-dioxane-ammonium hydroxide (concentrated solution) (30:68:2). Amitriptyline, fluoxetine and codeine R(f) medium values respectively are 0.82; 0.62; 0.42. Recommended methodology for the separation and identification of amitriptyline, fluoxetine and codeine in the mixture using thin-layer chromatography is statistically reliable: when confidence level is 0.95, relative error is less than 0.05; standard deviation is from 0.007 to 0.03. Recommended methodology suits for mixture, extracted from biological liquids, components separation and identification. ^{The Hive - Clandestine Chemists Without Borders}

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